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Comparison of pre-analytical conditions for quantification of serotonin in platelet-poor plasma. | LitMetric
Background : Reported concentrations of serotonin in platelet-poor plasma (PPP) in healthy subjects vary widely due to different pre-analytical procedures.Aim : To examine how different pre-analytical conditions affect the measured concentration of serotonin in PPP.Method : Six pre-analytical protocols were compared for preparation of PPP from EDTA whole blood for quantification of serotonin from nine healthy individuals. Three combinations of centrifugation with a mild centrifugation of gel-free EDTA tubes followed by a stronger centrifugation were compared to single-stage centrifugation of EDTA tubes with separator gel and heat shock treatment of blood prior to centrifugation. All samples were analysed using the same enzyme linked immunosorbent assay (ELISA) method.Results : Findings show that two consecutive centrifugations; first a mild centrifugation at 100 or 200× followed by centrifugation at 4500 or 14500× resulted in the lowest serotonin concentration in PPP.Conclusion : Two successive centrifugations to produce PPP for serotonin analysis; first a mild centrifugation to avoid mechanical stress on the platelets, and next a stronger centrifugation to remove platelets, is superior to the use of gel tubes and heat shock treatment.
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