AI Article Synopsis
- Intracellular bacteria like Brucella abortus must manage DNA damage caused by alkylating agents when they infect host cells, particularly in macrophages.
- The study shows that B. abortus relies on specific genes for DNA repair, highlighting the role of both direct and base-excision repair mechanisms during this stress.
- Notably, the gene ogt, essential for DNA repair, is regulated by the transcription factor GcrA, indicating unique features of DNA repair in B. abortus compared to well-studied bacteria like Escherichia coli.
Article Abstract
It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813329 | PMC |
| http://dx.doi.org/10.1038/s41467-019-12516-8 | DOI Listing |
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