AI Article Synopsis
- There is a growing need for a better way to measure how gold nanoparticles (AuNPs) are taken up by cells due to their many uses in biology.
- Currently, flow cytometry uses a 488 nm laser to detect AuNPs, but this method isn't very effective because it doesn't optimize the detection of these nanoparticles.
- The study shows that using lasers with a longer wavelength (red-shifted) significantly improves the detection of AuNPs, specifically in triple negative breast cancer cells.
Article Abstract
Due to the considerable amount of applications of gold nanoparticles (AuNPs) in biological systems, there is a great need for an improved methodology to quantitatively measure the uptake of AuNPs in cells. Flow cytometry has the ability to measure intracellular AuNPs by collecting the light scattering from a large population of live cells through efficient single cell analysis. Traditionally, the side scattering setting of the flow cytometer, which is associated with a 488 nm excitation laser (SSC channel), is used to detect nanoparticle uptake. This method is limited as AuNPs do not have the optimized response when excited with this laser. Here, we reported that the use of more red-shifted excitation lasers will greatly enhance the optical signal needed for the flow cytometry-based detection of AuNSs (26 nm in diameter) and AuNRs (67 nm × 33 nm, length × width) uptake in triple negative breast cancer cells (MDA-MB-231).
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| http://dx.doi.org/10.1021/acs.analchem.9b02248 | DOI Listing |
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