Determining transaminase activity in bacterial libraries by time-lapse imaging.

Chem Commun (Camb)

iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal.

Published: November 2019

Transaminase activity was determined by time-lapse imaging using a colourimetric reaction and image analysis. A correlation between the benzaldehyde conversion and relative luminance was determined, allowing the identification of the most promising biocatalysts, the determination of kinetic parameters, and the assessment of the effect of the substrate concentration on activity.

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Source
http://dx.doi.org/10.1039/c9cc07507kDOI Listing

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