AI Article Synopsis
- Ca, Na, and K ion channels, along with GPCRs (G-protein-coupled receptors) related to calcium release, are key targets in drug development.
- High-throughput fluorescence plate reader assays play a significant role in discovering and characterizing drugs that interact with these channels and receptors.
- The chapter covers the fundamental features of fluorescent dyes used in these assays and outlines methods for creating and optimizing fluorescence assays for studying ion channels and GPCRs.
Article Abstract
Ca, Na and K- permeable ion channels as well as GPCRs linked to Ca release are important drug targets. Accordingly, high-throughput fluorescence plate reader assays have contributed substantially to drug discovery efforts and pharmacological characterization of these receptors and ion channels. This chapter describes some of the basic properties of the fluorescent dyes facilitating these assay approaches as well as general methods for establishment and optimisation of fluorescence assays for ion channels and G-coupled GPCRs.
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| http://dx.doi.org/10.1007/978-3-030-12457-1_3 | DOI Listing |
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