AI Article Synopsis
- - The plant immune system has two main pathways: PAMP-triggered immunity, which detects general pathogen markers, and effector-triggered immunity, which senses specific proteins produced by pathogens.
- - Researchers studied how plants respond to the pathogen Xcc by analyzing gene expression changes at two time points after infection (3 and 12 days), finding more genes activated than suppressed, especially at 12 days.
- - The study emphasized the importance of timing in plant defenses and discovered the key role of two calcium-signaling proteins in resistance, confirmed by experiments with Arabidopsis knockout mutants.
Article Abstract
The plant immune system is divided into two branches; one branch is based on the recognition of pathogen-associated molecular patterns (PAMP-triggered immunity), and the other relies on pathogenic effector detection (effector-triggered immunity). Despite each branch being involved in different complex mechanisms, both lead to transcription reprogramming and, thus, changes in plant metabolism. To study the defense mechanisms involved in the - pv. (Xcc) interaction, we analyzed the plant transcriptome dynamics at 3 and 12 days postinoculation (dpi) by using massive analysis of 3'-cDNA ends. We identified more induced than repressed transcripts at both 3 and 12 dpi, although the response was greater at 12 dpi. Changes in the expression of genes related to the early infection stages were only detected at 12 dpi, suggesting that the timing of triggered defenses is crucial to plant survival. qPCR analyses in susceptible and resistant plants allowed us to highlight the potential role of two calcium-signaling proteins, and , in the resistance against Xcc. This role was subsequently confirmed using Arabidopsis knockout mutants.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6804691 | PMC |
| http://dx.doi.org/10.1038/s41438-019-0186-7 | DOI Listing |
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