Present work carried out with the objectives to isolate active component of and also to evaluate its free radical scavenging activity and preventing capacity against heavy metal toxicity. Solvents of different polarity were used to prepare crude extracts of seeds and screened for antioxidant activity. Among the crude extracts, methanolic extract was found to exhibit higher antioxidant activity (81.22%) which was fractionated by liquid-liquid partitioning method. Among the different fractions (LF1-LF4), LF-2 showed higher antioxidant activity (98.24%) as compared to other three liquid fractions and hence LF-2 was further purified by column chromatography. Among nine column fractions (CF1-CF9), fraction CF-7 was found to have higher antioxidant activity (92.14%), which was further analyzed using LC-MS and NMR and identified as loganic acid. radical scavenging assays showed remarkable antioxidant activity of loganic acid in terms of DPPH scavenging (IC 149 µg/ml), superoxide radical scavenging (IC 632.43 µg/ml) and hydroxyl radical scavenging (IC 29.78 µg/ml). Loganic acid exhibited 81% prevention of heavy metal toxicity through the mechanism of inhibiting ROS generation (2046 AU 5264 AU in control) and lipid peroxidation (95.01%). Thus, the active compound (loganic acid) isolated from has strong free radical scavenging activity and remarkable cyto-protective effect against heavy metal mediated toxicity.
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