Recently, it has been shown that water, confined in a plasma polymer subsurface chemical gradient, nanometers below the surface, significantly reduced the amount of adsorbed protein bovine serum albumin (BSA). Relating to this effect, we proposed the hypothesis that oriented water molecules within the subsurface gradient generate a long-range dipolar field, which interacts with dipolar proteins such as BSA near the surface region. This study extends the above used in situ multistep plasma deposition process to introduce plasma oxidation modifications of the subsurface architecture with the aim to further control the effect on protein adsorption. Neutron reflectivity measurements reveal that the oxidation time increases the amount of matrix-confined water. There is, however, an optimal oxidation time to obtain minimal protein adsorption, which suggests that a minimal distance between confined water molecules plays an important role. Altogether we can extend the range of controlling the adsorbed protein mass by the introduction of this additional plasma oxidation step.
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http://dx.doi.org/10.1021/acsami.9b14584 | DOI Listing |
Environ Pollut
January 2025
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Extracellular polymeric substances (EPS) can effectively attenuate heavy metal mobility in aquatic ecosystems and reduce metal toxicity to cells. However, a systematic study of microalgae EPS responses and their adsorption behaviors, characteristics, and mechanisms under different heavy metal exposures has not been performed. In this study, EPS extracted from Chlamydomonas reinhardtii CC-125 was analyzed for compositional changes (monosaccharides and proteins) under Cd, Cu, Pb, and Zn treatments.
View Article and Find Full Text PDFJ Phys Chem Lett
January 2025
Department of Physics, Chalmers University of Technology, 412 96 Göteborg, Sweden.
Functional gold nanoparticles have emerged as a cornerstone in targeted drug delivery, imaging, and biosensing. Their stability, distribution, and overall performance in biological systems are largely determined by their interactions with molecules in biological fluids as well as the biomolecular layers they acquire in complex environments. However, real-time tracking of how biomolecules attach to colloidal nanoparticles, a critical aspect for optimizing nanoparticle function, has proven to be experimentally challenging.
View Article and Find Full Text PDFACS Omega
January 2025
Shaanxi University of Chinese Medicine, Xianyang 712046, China.
Research on bone substitutes for repairing bone defects has drawn increasing attention, and the efficacy of three-dimensional (3D) printed bioactive porous scaffolds for bone defect repair has been well documented. Our previous studies have shown that psoralen can promote osteogenesis by activating the Wnt/β-catenin and BMP/Smad signaling pathways and their crosstalk effects, and psoralen nanospheres have a good osteogenesis-promoting effect with low cytotoxicity. The Chinese medicine oyster shell powder, characterized by its porous structure, strong adsorption, and unique bioactivity, has potential in fracture-promoting repair materials.
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January 2025
Federal University of Espírito Santo, Av Marechal Campos 1468, Vitória, ES 29.040 090, Brazil.
Monodisperse and colloidally stable magnetic iron oxide nanoparticles have been developed for diverse biotechnology applications. Although promising for the adsorption of organic molecules, the low density of adsorption sites in these nanoparticles has been a significant challenge. In this study, an optimized factorial design with response surface methodology (RSM) was employed to produce small Superparamagnetic Iron Oxide Nanoparticles (SPIONs) stabilized with tetraethoxysilane (TEOS).
View Article and Find Full Text PDFLangmuir
January 2025
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
As one of the key diagnostic methods for detecting biomarkers and antigen-antibody interactions, the luminescent oxygen channel immunoassay (LOCI) has been widely applied in bioanalysis and other fields. In the context of LOCI, the performance of the prepared donor polystyrene (PS) microspheres significantly impacts the detection signal values. In this study, an attempt was made to synthesize PS microspheres via one-step polymerization of styrene with an amphiphilic monomer (PEOOH), followed by swelling the silicon phthalocyanine photosensitizer into the PS microspheres, resulting in the functionalization of the PS microspheres with polyethylene glycol segments.
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