Cas9 has no exonuclease activity resulting in staggered cleavage with overhangs and predictable di- and tri-nucleotide CRISPR insertions without template donor.

Xin Shi Jia Shou Mohammadreza M Mehryar Jingwei Li Leyang Wang Mo Zhang Haiyan Huang Xiaofang Sun Qiang Wu

Cell Discov

1Center for Comparative Biomedicine, MOE Key Lab of Systems Biomedicine, State Key Lab of Oncogenes and Related Genes, Shanghai Cancer Institute, Joint International Research Laboratory of Metabolic & Developmental Sciences, Institute of Systems Biomedicine, Xin Hua Hospital, Shanghai Jiao Tong University, Shanghai, China.

Published: October 2019

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6796948	PMC
http://dx.doi.org/10.1038/s41421-019-0120-z	DOI Listing

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