AI Article Synopsis
- The study focused on producing silver nanoparticles (AgNPs) using a specific actinobacterial strain (AS-3) isolated from lawn soil, identified through gene sequencing.
- Characterization of the synthesized AgNPs was performed using various techniques, revealing spherical nanoparticles with an average size of 22 nm and confirming the presence of silver and proteins in their structure.
- The AgNPs exhibited broad-spectrum antibacterial activity, demonstrated through disc diffusion tests and minimum inhibitory concentration (MIC) analysis.
Article Abstract
The aim of this study is to fabricate silver nanoparticles (AgNPs) using actinobacterial strain isolated from lawn soil. Among six isolates, one isolate named AS-3 was potent in AgNPs production; hence it was identified deployed on gene sequence (16S rRNA) as AS-3 (99.8% similarity). Actinobacteria mediated synthesized AgNPs were analyzed using UV-visible spectroscopy (UV-Vis), which showed a Surface Plasmon Resonance (SPR) at around = 443 nm. Scanning electron microscopy (SEM) analyses revealed the occurrence of predominant spherical AgNPs with polydispersed, with an average size of 22 nm. Energy-dispersive X-ray spectroscopy (EDS) established the existence of silver component. While the Fourier transforms infrared spectroscopy (FTIR) evidenced the occurrence of proteins as the bio reduction and topping agents over the AgNPs. X-ray diffraction (XRD) examination confirmed the obtained AgNPs were in crystalline planes of the face centric cubic. The AgNPs antibacterial activity showed a broad spectrum antibacterial action against sp., and were confirmed by disc diffusion test and MIC analysis.
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| http://dx.doi.org/10.1166/jnn.2020.17440 | DOI Listing |
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