Molecular typing of bacterial isolates provides a powerful approach for distinguishing genotypes. It is known that strain virulence plays a role in prevalence and spread of the disease, suggesting that strain virulence and prevailing genotypes are associated. However, it is not well understood whether strain virulence correlates with particular genotypes. In this study, we assessed the in vitro intracellular growth of 18 isolates in bovine macrophages as an indicator of bacterial virulence and sought a relationship with the genotype identified by spoligotyping. We found 14 different spoligotypes-11 were already known and three spoligotypes had never been reported before. We identified 2 clusters that were phylogenetically related, containing 10 and 6 strains, respectively, and 2 orphan strains. Intracellular growth and phagocytic rates of 18 strains were heterogeneous. Our results suggest that intracellular growth and phagocytosis are independent of the bacterial lineage identified by spoligotyping.
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| http://dx.doi.org/10.3390/vetsci6040081 | DOI Listing |
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