[Effect of Huntingtin-associated Protein 1 Gene on Proliferation of Mouse Fibroblasts].

Objective: To determine the effect of Huntingtin-associated protein 1 ( 1) on fibroblast proliferation.

Methods: 1 knockout ( 1 ) primary fibroblasts were isolated and cultured . The proliferation of 1 fibroblasts was detected by EdU proliferation assay and cell flow assay. Transcriptome sequencing of the wild-type and 1 fibroblasts was screened for proliferation-related genes. Real-time quantitative PCR (qPCR) was performed to verify changes in expressions of related genes. Skin repair was examined in 1 knockdown mice with skin wounds. The proliferation of fibroblasts during wound repair was detected by PCNA immunohistochemical staining.

Results: 1 fibroblasts were successfully cultured. Compared with WT, EdU-positive fibroblasts decreased in 1 ,with less cells entering the S phase. Transcriptome sequencing of primary fibroblasts identified genes of 25, 27, 28 and 5. qPCR confirmed that 1 knockout increased 27 expression. 1 mice had larger skin lesions, slower healing and lower positive density of fibroblast proliferation than those of wild type mice.

Conclusion: 1 may positively regulate fibroblast proliferation by inhibiting the expression of cell cycle negative regulator 27.Its deletion inhibits fibroblasts entering the S phase, thereby reducing cell proliferation and affecting wound repair.