Osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (BMSCs) has been regarded as a central issue in fracture healing. MicroRNAs (miRNAs, miRs) participate in diverse physiological processes such as osteoblastic differentiation of BMSCs. In this study, we found that miR-664a-5p was upregulated during osteogenic differentiation of human BMSCs, and this upregulation positively correlated with the expression of osteogenic genes Runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN). Overexpression of miR-664a-5p promoted the osteogenic differentiation of BMSCs, whereas a knockdown of miR-664a-5p suppressed it. Additionally, high-mobility group A2 (HMGA2) mRNA was identified as a direct target of miR-664a-5p that mediates the function of this miRNA. Overexpression of HMGA2 obviously attenuated miR-664a-5p-induced osteogenic differentiation of BMSCs. Thus, the newly identified miR-664a-5p-HMGA2 pathway expands our understanding of the mechanisms underlying the osteogenic differentiation of human BMSCs, may provide deeper insights into the regulation of this differentiation, and can point to new effective methods for treating osteoporosis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.bbrc.2019.09.122 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Surface engineering of 3D-printed polylactic acid scaffolds with polydopamine and 4-methoxycinnamic acid-chitosan nanoparticles for bone regeneration.
Nanoscale Adv
January 2025
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology Kattankulathur Tamil Nadu 603203 India
Bone remodeling, a continuous process of resorption and formation, is essential for maintaining skeletal integrity and mineral balance. However, in cases of critical bone defects where the natural bone remodeling capacity is insufficient, medical intervention is necessary. Traditional bone grafts have limitations such as donor site morbidity and availability, driving the search for bioengineered scaffold alternatives.
View Article and Find Full Text PDFCitrate: a key signalling molecule and therapeutic target for bone remodeling disorder.
Front Endocrinol (Lausanne)
January 2025
Department of Orthopedics, The First Hospital of China Medical University, Shenyang, China.
Bone remodeling is a continuous cyclic process that maintains and regulates bone structure and strength. The disturbance of bone remodeling leads to a series of bone metabolic diseases. Recent studies have shown that citrate, an intermediate metabolite of the tricarboxylic acid (TCA) cycle, plays an important role in bone remodeling.
View Article and Find Full Text PDFChiral polymer-induced hydroxyapatite for promoting bone regeneration.
Mater Today Bio
April 2025
Cancer Institute, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, China.
Chirality is one of the basic characteristics of living matter, yet the effect of chiral polymers on osteogenesis is seldom studied. Thus, it is necessary to deeply recognize the behaviors of chiral polymers in osteogenic processes, which can be beneficial for the development of bone repair materials. In this work, chiral hydroxyapatite (HAP) was constructed simply using poly(levorotatory/dextral-tartaric acid) as the guest of the chiral transfer system.
View Article and Find Full Text PDFIonic silver coating of orthopedic implants may impair osteogenic differentiation and mineralization.
Exp Ther Med
March 2025
OrthoLab, The Rudbeck Laboratory, Department of Surgical Sciences/Orthopedics, Uppsala University, 75185 Uppsala, Sweden.
Silver (Ag) possesses potent antimicrobial properties and is used as a coating for medical devices. The impact of silver ions released from orthopedic implants on the differentiation and osteoid formation of different osteogenic cells has yet to be systematically studied. In the present study, human mesenchymal stem cells (hMSCs) and primary human osteoblasts (hOBs) were exposed to different static Ag concentrations (0, 0.
View Article and Find Full Text PDFEu-Doped TiO Coatings One-Step Preparation Enhance Macrophage Polarization and Osseointegration of Implants.
ACS Appl Mater Interfaces
January 2025
College of Civil Engineering and Mechanics of Lanzhou University, Lanzhou 730000, China.
The controllable regulation of immune and osteogenic processes plays a critical role in the modification of biocompatible materials for tissue regeneration. In this study, titanium dioxide-europium coatings (MAO/Eu) were prepared on the surface of a titanium alloy (Ti-6Al-4V) a one-step process combining microarc oxidation (MAO) and doping. The incorporation of Eu significantly improved the hydrophilic and mechanical properties of the TiO coatings without altering their morphology.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!