Background: A T cell subtype with the CD4CD25FoxP3 phenotype was recently described. We aimed to investigate the frequency of these cells and their ability to produce cytokines in tumor-draining lymph nodes from patients with breast cancer (BC).
Materials And Methods: Mononuclear cells from lymph nodes of 20 patients with BC were activated and stained for appropriate markers. The cells were assayed with four-color flow cytometry.
Results: A very small fraction of CD4CD25FoxP3 cells produced cytokines at levels that were significantly lower than in the regulatory (CD4CD25FoxP3) and effector cell (CD4CD25FoxP3) subpopulations. The expression of IFNγ and IL-2 in the CD4CD25FoxP3 subset was significantly higher than in Treg cells, but lower than in the effector subset. Conversely, IL-22 expression in Treg cells was significantly higher than in the CD4CD25FoxP3 subpopulation. The expression of IL-10 in the CD4CD25FoxP3 subset was also significantly higher than in effector cells.
Conclusion: We suggest that CD4CD25FoxP3 cells in patients with BC are exhausted cells with an intermediate phenotype between effector and regulatory cells.
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