AI Article Synopsis
- Two TaqMan® qPCR assays were created to accurately measure the amounts of two specific algae, Scenedesmus obliquus and Chlorella vulgaris, by targeting the psbA gene.
- The assays showed high amplification efficiencies (92.4% for S. obliquus and 96.6% for C. vulgaris) and strong correlation values (R = 0.99), suggesting reliable results.
- These assays can effectively quantify these algae in biofilms, aiding in better management of algal cultivation for biofuels and other bioproducts.
Article Abstract
Two TaqMan® qPCR assays were developed to specifically quantify the absolute abundance of Scenedesmus obliquus and Chlorella vulgaris in mixed-species algal biofilms by targeting the psbA gene. Standard curves were developed with amplification efficiencies of 92.4% and 96.6% for S. obliquus and C. vulgaris, respectively, and an R value of 0.99 for both. Calibration curves for estimating absolute cell abundances resulted in slopes of 0.98 and 1.11 for C. vulgaris and S. obliquus, respectively, and an R value of 0.95 for both. The assays were applied to cultivated mixed-species biofilms and approximately 10 cells of each algal species were quantified when 10 cells were added into biofilms. The developed qPCR assays were concluded to be specific and accurate for the quantification of S. obliquus and C. vulgaris in mixed-species biofilms. This will contribute to the control and optimization of algal cultivation systems for the production of algal biofuels and bioproducts.
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| http://dx.doi.org/10.1016/j.biortech.2019.122251 | DOI Listing |
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