The genes from MSB03 were cloned and expressed to investigate binding ability on clay minerals and sea sand at pH ranging 3 to 9. FTIR analysis has been done to characterize bound enzymes on clay minerals. Subsequent, surveying of NCBI database for extracellular enzymes of soil bacteria was carried out. Among the five cloned Cel enzymes assayed for binding to clay minerals, only Cel5H enzyme had the binding ability. Enzyme Cel5H exhibited highest binding to montmorillonite followed by kaolinite and sea sand. Interestingly, Cel5H had higher pI value of 9.24 than other proteins (5.2-5.7). Cel5H binding to montmorillonite was shown to be negatively affected below pH 3 and above pH 9. Infrared absorption spectra of the Cel5H-montmorillonite complexes showed distinct peaks for clay minerals and bound proteins. Furthermore, database survey of soil bacterial extracellular enzymes revealed that species enzymes had higher pI than other soil bacterial enzymes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/09168451.2019.1679613 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Spatial distribution and driving factors of soil selenium on the Leizhou Peninsula, southern China.
Environ Geochem Health
January 2025
Institute of Geophysical and Geochemical Exploration, Chinese Academy of Geological Sciences, No. 84, Jinguang Road, Langfang, 065000, China.
Selenium (Se) is an essential element for humans, playing a critical role in the functioning of the immune system. The global prevalence of dietary Se deficiency is a significant public health concern, largely attributed to the low levels of Se present in crops. The sufficient Se in plants and humans is determined by the presence of stable Se sources in the soil.
View Article and Find Full Text PDFA selective non-enzymatic synthesis of ribose simply from formaldehyde, metal salts and clays.
Chem Commun (Camb)
January 2025
School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210023, P. R. China.
This study demonstrates that metal-doped-clay (MDC) can be a selective platform for ribose produced from formaldehyde under abiotic conditions. Ribose exhibits superior retention compared with other carbohydrates on naturally occurring minerals on the early Earth in the presence of divalent cations. This finding offers an insight into the necessity of the emergence of ribose as the backbone of extant RNA.
View Article and Find Full Text PDFHyperspectral inversion of heavy metal content in farmland soil under conservation tillage of black soils.
Sci Rep
January 2025
College of Jilin Emergency Management, Changchun Institute of Technology, Changchun, 130012, China.
Globally, heavy metal (HM) soil pollution is becoming an increasingly serious concern. Heavy metals in soils pose significant environmental and health risks due to their persistence, toxicity, and potential for bioaccumulation. These metals often originate from anthropogenic activities such as industrial emissions, agricultural practices, and improper waste disposal.
View Article and Find Full Text PDFEnhanced remediation of heavy metal-contaminated soils using biochar and zeolite combinations with additives: A meta-analysis.
Environ Pollut
December 2024
Faculty of Agronomy and Veterinary Medicine, University of Brasília, 70910-970, Brasília, DF, Brazil. Electronic address:
Article Synopsis
- Soil heavy metal contamination in agricultural lands poses health risks through potential entry into the food chain, prompting research on remediation materials like biochar (BC) and zeolites (ZE).
- Both BC and ZE individually reduce the availability of heavy metals such as cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn), with BC achieving reductions of 24% to 10% and ZE achieving reductions ranging from 32% to 39%.
- The combination of BC and ZE further enhances these reductions, and when paired with certain inorganic additives, the effectiveness against Cd increases significantly, demonstrating that the choice of additive plays a crucial role in the remediation process.
Catalytic and Noncatalytic in Situ Hydrogen Production from Heavy Oil: A Review of Experimental Studies.
ACS Omega
December 2024
Department of Petroleum Engineering, King Fahd University of Petroleum & Minerals (KFUPM), Dhahran 31261, Saudi Arabia.
Hydrogen (H) offers a less carbon-intensive energy production method than natural gas. The potential of utilizing hydrogen at a large scale within the future energy mix to fuel the world opens the door to investigating hydrogen production from heavy and extra-heavy oil reservoirs. Various reaction mechanisms are involved in the in situ combustion gasification of heavy oil to produce sustainable and low carbon intensive hydrogen.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!