Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum.

The aim of this study was to test and compare two new components in extenders for freezing donkey semen: mare colostrum and jenny colostrum. Colostrum was obtained from four mares and four jennies right after the foal's birth. Ejaculates were collected from five fertile donkeys. Sperm samples were pooled, diluted and cryopreserved in three different experimental extender groups: lactose supplemented with egg yolk extender (20%) as the control group, lactose supplemented with jenny colostrum extender (20%), and lactose supplemented with mare colostrum extender (20%). After thawing, we evaluated the sperm motility by means of computer-assisted analysis, viability by SYBR-14 and propidium iodide (PI), membrane functional by HOS test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC-PNA) and PI. The results demonstrated that lactose-jenny colostrum extender displayed significantly higher values (p < .05) in nearly all parameters evaluated - Total Motility, Viability, HOS test, VCL, VSL, VAP, LIN, STR and WOB -, compared with mare colostrum and egg yolk extenders after thawing. In conclusion, the extender containing jenny colostrum used for donkey semen cryopreservation improved the donkey sperm quality after the freezing-thawing process.