AI Article Synopsis
- A new bacterium strain, identified as M30, was isolated from Lake Magadi in Tanzania and is characterized as alkaliphilic, moderately halophilic, and heterotrophic.
- This rod-shaped, spore-forming bacterium thrives in aerobic conditions and grows best at temperatures of 25-30°C, a pH range of 9.5-10, and salinity levels around 5%.
- Biochemical tests revealed the strain's ability to utilize various sugars and organic acids, produce enzymes like amylase and protease, and its genetic analysis affiliated it closely with other specific bacterial strains, leading to its classification as a novel species.
Article Abstract
An alkaliphilic, moderately halophilic, heterotrophic, rod-shaped, spore-forming bacterium (M30) was isolated from a sediment sample collected from a soda lake (Lake Magadi, Tanzania). Strain M30 was strictly aerobic, catalase-positive, oxidase-negative and non-motile. Growth occurred at 12-43 °C (optimum, 25-30 °C), at pH 8.0-12 (optimum, pH 9.5-10) and at salinities of 0.5-15 % (w/v) NaCl (optimum 5 %). It utilized various sugars and organic acids as sole carbon sources and was positive for amylase, cellulase, gelatinase, protease and xylanase activities. The cell-wall peptidoglycan contained -diaminopimelic acid and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid and one unidentified phospholipid. The DNA G+C content was 48.9 mol%. The predominant menaquinone was MK-7 and the major fatty acids (>10 %) comprised anteiso-C, iso-C, and anteiso-C. Phylogenetic analysis based on 16S rRNA gene sequence affiliated M30 to the genus and showed the highest similarities to FJAT-45347 (96.4 %) and K1-5 (96.2 %). Based on the data from the current polyphasic study, M30 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M30 (=JCM 32118=CGMCC 1.16739=MCC 3010).
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| http://dx.doi.org/10.1099/ijsem.0.003792 | DOI Listing |
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