sp. nov., from a mixed hydrogen-oxidizing bacteria enrichment reactor for microbial protein production.

Strain LMG 30378 was isolated from a hydrogen-oxidizing bacteria enrichment reactor inoculated with forest soil. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that this strain belonged to the genus . Multilocus sequence analysis combined with sequence analysis of a 765 bp gene fragment both showed LMG 3411 and LMG 1231 to be the closest-related neighbours to strain LMG 30378. Genome sequence analysis revealed a draft genome of 6.81 Mb with a G+C content of 67.2 mol%. DNA-DNA hybridization with LMG 1231 and LMG 3411 showed 42.7 and 42.5% similarity, respectively, confirming that strain LMG 30378 represented a novel species. Phenotypic and metabolic characterization revealed acid phosphatase activity and the absence of phosphoamidase activity as distinctive features. The draft genome composes all necessary metabolic components to fix carbon dioxide and to oxidize molecular hydrogen, suggesting that strain LMG 30378 is a key organism in the enrichment reactor. Together, these data demonstrate that strain LMG 30378 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LMG 30378 (=CCUG 71558).