Detection of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), is highly reliant on traps loaded with trimedlure (TML), a male-specific attractant. The successful application of attractants requires that constituents are placed in a suitable dispensing system to reduce volatilization and increase the effective field longevity of the attractant. A series of trials were conducted in different fruit orchards to test 1) the efficiency of a polyethylene-matrix plug in regulating the emission rate of TML 2) and the lifetime attractiveness of the plug compared to the cotton wick dispenser. Cotton dispensers containing the recommended dose of TML (0.75 cm3) and with a double dose (1.5 cm3) remained active for 6 and 8 wk, respectively. The increase in TML dose from 0.75 cm3 to 1.5 cm3 led to an increase in the longevity of the cotton dispenser, but there was no significant difference between the doses in biological activity. In all trials, TML-polyethylene matrix dispensers were more efficient under field conditions as they attracted more males for up to 12 wk and prolonged the release of TML more than the cotton wick dispensers. The TML-loaded polyethylene matrix dispensers might be useful in 1) minimizing the losses of the liquid TML from cotton wicks, 2) avoiding the damage that occurs when using other dispensers, 3) prolonging the TML lifetime in the field through regulating its release rates, 4) potentially minimizing the adverse effect of high temperatures on release rate, and, finally, 5) minimizing the costs of using large amounts of expensive TML solutions.
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http://dx.doi.org/10.1093/jee/toz275 | DOI Listing |
Nat Commun
December 2024
Research Center for Applied Sciences, Academia Sinica, Taipei, 11529, Taiwan.
Taking advantage of the good mechanical strength of expanded Drosophila brains and to tackle their relatively large size that can complicate imaging, we apply potassium (poly)acrylate-based hydrogels for expansion microscopy (ExM), resulting in a 40x plus increased resolution of transgenic fluorescent proteins preserved by glutaraldehyde fixation in the nervous system. Large-volume ExM is realized by using an axicon-based Bessel lightsheet microscope, featuring gentle multi-color fluorophore excitation and intrinsic optical sectioning capability, enabling visualization of Tm5a neurites and L3 lamina neurons with photoreceptors in the optic lobe. We also image nanometer-sized dopaminergic neurons across the same intact iteratively expanded Drosophila brain, enabling us to measure the 3D expansion ratio.
View Article and Find Full Text PDFNat Commun
December 2024
Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8577, Japan.
Protein is essential for all living organisms; however, excessive protein intake can have adverse effects, such as hyperammonemia. Although mechanisms responding to protein deficiency are well-studied, there is a significant gap in our understanding of how organisms adaptively suppress excessive protein intake. In the present study, utilizing the fruit fly, Drosophila melanogaster, we discover that the peptide hormone CCHamide1 (CCHa1), secreted by enteroendocrine cells in response to a high-protein diet (HPD), is vital for suppressing overconsumption of protein.
View Article and Find Full Text PDFGenetics
December 2024
Department of Genetics and Biochemistry and Center for Human Genetics, Clemson University, 114 Gregor Mendel Circle, Greenwood, SC 29646, USA.
Mucopolysaccharidosis type IIIB (MPS IIIB) is a rare lysosomal storage disorder caused by defects in alpha-N-acetylglucosaminidase (NAGLU) and characterized by severe effects in the central nervous system. Mutations in NAGLU cause accumulation of partially degraded heparan sulfate in lysosomes. The consequences of these mutations on whole genome gene expression and their causal relationships to neural degeneration remain unknown.
View Article and Find Full Text PDFEcol Lett
January 2025
Department of Biology, University of Oxford, Oxford, UK.
Interactions between species pose considerable challenges for forecasting the response of ecological communities to global changes. Coexistence theory could address this challenge by defining the conditions species can or cannot persist alongside competitors. However, although coexistence theory is increasingly deployed for projections, these frameworks have rarely been subjected to critical multigenerational validation tests.
View Article and Find Full Text PDFSleep Adv
December 2024
Murine Phenotyping Core, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
Model organisms such as are powerful tools to study the genetic basis of sleep. Previously, we identified the genes and using selective breeding for long and short sleep duration in an outbred population of . is a transcription factor that is part of the epidermal growth factor receptor signaling pathway, while is involved in proline and arginine metabolism.
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