An ultrasonication-promoted strategy was proposed to synthesize luminescent S-dots, which reduced the synthesis time from the commonly used 5 days to several hours. The as-synthesized S-dots show a high photostability and low cytotoxicity, and are then successfully applied for cellular imaging.
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http://dx.doi.org/10.1039/c9cc06586e | DOI Listing |
PLoS One
December 2024
School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.
Immunofluorescence is highly dependent on antibody-antigen interactions for accurate visualization of proteins and other biomolecules within cells. However, obtaining antibodies with high specificity and affinity for their target proteins can be challenging, especially for targets that are complex or naturally present at low levels. Therefore, we developed AptaFluorescence, a protocol that utilizes fluorescently labeled aptamers for in vitro biomolecule visualization.
View Article and Find Full Text PDFMAGMA
December 2024
Department of Radiology and Medical Informatics, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Objectives: Phosphorus-31 magnetic resonance spectroscopic imaging (P-MRSI) is a non-invasive tool for assessing cellular high-energy metabolism in-vivo. However, its acquisition suffers from a low sensitivity, which necessitates large voxel sizes or multiple averages to achieve an acceptable signal-to-noise ratio (SNR), resulting in long scan times.
Materials And Methods: To overcome these limitations, we propose an acquisition and reconstruction scheme for FID-MRSI sequences.
Cell Tissue Bank
December 2024
Division of Shoulder and Elbow Surgery, Rothman Orthopaedic Institute, Philadelphia, PA, USA.
Tissue engineering and cartilage transplantation constitute an evolving field in the treatment of osteoarthritis, with therapeutic and clinical promise shown in autologous chondrocyte implantation. The aim of this systematic review is to explore current clinical trials that utilized autologous chondrocyte transplantation (ACT) and assess its efficacy in the treatment of osteoarthritis. PubMed, Ovid MEDLINE, and Google-Scholar (pages 1-20) were searched up until February 2023.
View Article and Find Full Text PDFEur J Nucl Med Mol Imaging
December 2024
Department of Radiation Oncology, Stanford University, Stanford, CA, USA.
Purpose: Nanoparticles are highly efficient vectors for ferrying contrast agents across cell membranes, enabling ultra-sensitive in vivo tracking of single cells with positron emission tomography (PET). However, this approach must be fully characterized and understood before it can be reliably implemented for routine applications.
Methods: We developed a Langmuir adsorption model that accurately describes the process of labeling mesoporous silica nanoparticles (MSNP) with Ga.
Anal Bioanal Chem
December 2024
Faculty of Science and Technology, Seikei University, 3-3-1 Kichijoji-Kitamachi, Musashino, Tokyo, 180-8633, Japan.
Methods that facilitate molecular identification and imaging are required to evaluate drug penetration into tissues. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), which has high spatial resolution and allows 3D distribution imaging of organic materials, is suitable for this purpose. However, the complexity of ToF-SIMS data, which includes nonlinear factors, makes interpretation challenging.
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