AI Article Synopsis
- The FluChip-8G Insight assay is a new tool developed for quick identification and subtyping of seasonal and potential pandemic influenza viruses in a single test, providing results on the same day.
- The study aimed to validate the performance of this assay by analyzing a variety of non-seasonal influenza A strains, and it was tested on 297 samples from 180 unique strains.
- Results showed that the assay had a high positive agreement (≥ 93%) for most subtypes, indicating it is effective for detecting various influenza viruses, with the added benefit of being easily updatable for new strains.
Article Abstract
Background: Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip-8G Insight assay was developed to subtype both seasonal and potentially pandemic influenza viruses in a single assay with a same day result. FluChip-8G Insight uses whole gene segment RT-PCR-based amplification to provide robustness against genetic drift and subsequent microarray detection with artificial neural network-based data interpretation.
Objectives: The objective of this study was to verify and validate the performance of the FluChip-8G Insight assay for the detection and positive identification of human and animal origin non-seasonal influenza A specimens.
Methods: We evaluated the ability of the FluChip-8G Insight technology to type and HA and NA subtype a sample set consisting of 297 results from 180 unique non-seasonal influenza A strains (49 unique subtypes).
Results: FluChip-8G Insight demonstrated a positive percent agreement ≥93% for 5 targeted HA and 5 targeted NA subtypes except for H9 (88%), and negative percent agreement exceeding 95% for all targeted subtypes.
Conclusions: The FluChip-8G Insight neural network-based algorithm used for virus identification performed well over a data set of 297 naïve sample results, and can be easily updated to improve performance on emerging strains without changing the underlying assay chemistry.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928037 | PMC |
| http://dx.doi.org/10.1111/irv.12683 | DOI Listing |
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Article Synopsis
- The FluChip-8G Insight assay is a new tool developed for quick identification and subtyping of seasonal and potential pandemic influenza viruses in a single test, providing results on the same day.
- The study aimed to validate the performance of this assay by analyzing a variety of non-seasonal influenza A strains, and it was tested on 297 samples from 180 unique strains.
- Results showed that the assay had a high positive agreement (≥ 93%) for most subtypes, indicating it is effective for detecting various influenza viruses, with the added benefit of being easily updatable for new strains.
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