AI Article Synopsis
- The novel fluorescent probe HPQRB is designed for detecting sulfite in living HepG2 cells using an ESIPT effect, enabling sensitive and rapid measurements.
- HPQRB is synthesized through a straightforward two-step condensation reaction, resulting in significant shifts in fluorescence emission that enhance imaging capabilities.
- The probe exhibits high selectivity for sulfite, responding quickly within 30 seconds, and has successfully demonstrated effective fluorescence imaging of sulfite in physiological conditions with a low detection limit of 44 nM.
Article Abstract
The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two-step condensation reaction. HPQRB has a large emission shift (Δ=116 nm), which is beneficial for fluorescence imaging research, and its sulfite-responsive site is based on a rhodamine-like structure with the emission peak at 566 nm, which decreases with increasing sulfite concentration. and its HPQ structure always has an ESIPT effect throughout the reaction process, keeping the emission peak at 450 nm as a self-reference. In particular, HPQRB has high selectivity for sulfite and responds quickly (within 30 s) with a low detection limit (44 nM). Furthermore, HPQRB has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6779067 | PMC |
| http://dx.doi.org/10.1002/open.201900242 | DOI Listing |
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