Purpose: Piezo-assisted intracytoplasmic sperm injection (Piezo-ICSI) is reported to be an effective method for inseminating fragile oocytes compared with conventional ICSI (c-ICSI). However, infertile patient groups suitable for Piezo-ICSI have not been elucidated. This study was conducted to determine age groups suitable for Piezo-ICSI using sibling egg controls inseminated by a well-trained embryologist to reduce technical inequalities.
Methods: A total of 947 matured oocytes were inseminated either by Piezo-ICSI or by c-ICSI in sibling oocytes as controls. Fertilization (2 pronuclei, PN), survival, and blastocyst development rates on day (D) 5 and D6 after insemination were compared between the Piezo-ICSI and c-ICSI groups. Further analyses were applied to groups of women 35 or ≤35 years of age.
Results: There were no significant differences in fertilization, survival, or blastocyst development rates between the two insemination treatment groups. However, for women 35 years of age, the fertilization ( = .008) and blastocyst development ( = .016) rates with Piezo-ICSI on D5 and D6 were significantly higher than in those subjected to c-ICSI.
Conclusions: Piezo-ICSI was useful for inseminating oocytes from women >35 years of age.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6780025 | PMC |
| http://dx.doi.org/10.1002/rmb2.12290 | DOI Listing |
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