Tomato bacterial canker caused by subsp. is one of the most important seed-borne tomato diseases around the globe. The disease was initially reported in 1993 in Iran, and it became a rising threat for the multibillion dollar tomato industry of the country during the last decade. In this study, using phylogeographic analyses, we determined genetic diversity and geographic distribution of subsp in Iran. Our field surveys showed that the pathogen is expanding into the southern and eastern areas of the country. Furthermore, multilocus sequence analysis and typing (MLSA/MLST) using the sequences of five housekeeping genes (, , , , and ) revealed that 37 subsp strains isolated in Iran had high genetic diversity and placed in 15 sequence types (STs), while all the available 184 worldwide subsp sequences were placed in 43 STs. MLSA divided the worldwide subsp strains into two phylogroups (I and II). Among the 37 strains isolated in Iran, 30 strains clustered in phylogroup I, while 7 strains clustered in phylogroup II. Phylogeographic data inferred from the allelic profile of the five housekeeping genes suggested multiple introductions of subsp inoculum into Iran, while the geographic origin of the Iranian subsp strains remains undetermined. Further analyses using higher numbers of strains are warranted to decipher the evolutionary history of subsp in Iran. Additionally, stricter seed/transplant inspections are recommended to reduce the risk of pathogen expansion to areas with no history of the disease. subsp. , the causal agent of tomato bacterial canker disease, is one of the economically important pathogens of solanaceous crops (e.g., eggplant, pepper, and tomato) around the world. The disease occurs in many countries, with a particular importance in regions characterized by high precipitation and humid environmental conditions. As a seed-borne pathogen, subsp is included in the A2 (high risk) list of quarantine pathogens by the European and Mediterranean Plant Protection Organization (EPPO). Bacterial canker disease was reported for the first time in 1993 in Iran, while the geographic distribution, genetic diversity, and phylogenetic position of the causal agent remain undetermined. In this study, using the multilocus sequence analysis and typing (MLSA/MLST) approach, we provided a phylogeographic scheme for the subsp strains isolated in Iran. Furthermore, global-scale phylogenetic analyses led to determination of phylogenetic position of Iranian subsp strains among worldwide population of the pathogen. Based on diversity parameters and population structure, we suggest relatively higher genetic diversity of the bacterial canker pathogen in Iran than has so far been observed in the other areas of the world. Results obtained in this study provide a novel insight into the genetic diversity and population structure of the bacterial canker pathogen on a global scale.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6881797 | PMC |
http://dx.doi.org/10.1128/AEM.02098-19 | DOI Listing |
Plant Physiol Biochem
December 2024
Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Science and Technology Research on Fruit Tree, Guangzhou, 510640, China. Electronic address:
Citrus bacterial canker has deleterious effects on global citrus production. The mitogen-activated protein kinase (MAPK) signaling cascade regulates plant defense against pathogen infection. Here, we identified 11 MAPKs in Atalantia buxifolia, a wild citrus species with high stress tolerance.
View Article and Find Full Text PDFJ Appl Microbiol
December 2024
VBlab - Laboratory of Bacterial Viruses, University of Sorocaba, 18023-000 Sorocaba/SP, Brazil.
Aims: In this study we report the use of two novel lytic polyvalent phages as a cocktail in in planta assays and its efficacy in the control of bacterial halo blight (BHB) caused by Pseudomonas coronafaciens pv. garcae (Pcg) in coffee plants.
Methods And Results: Phages were isolated from samples of coffee plant leaves collected at two different locations in Brazil.
Phytopathology
December 2024
University of Tehran, Department of Plant Protection,, University of Tehran, Tehran, Iran (the Islamic Republic of), 31587-77871;
Bacterial canker of tomato caused by the Gram-positive corynebacterial species is one of the most destructive seed-borne diseases in both open air and greenhouse tomatoes. The pathogen is a regulated agent in all tomato-producing countries as translocation of infected tomato materials transports the bacterium into new areas. is generally known to have yellow-pigmented colonies on culture media, which is a key differentiative phenotypic feature in standard diagnostic guidelines.
View Article and Find Full Text PDFJ Nanobiotechnology
November 2024
State Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals of Guizhou University, Guiyang, 550025, China.
Background: Bacterial diseases caused by phytopathogenic Xanthomonas pose a significant threat to global agricultural production, causing substantial economic losses. Biofilm formation by these bacteria enhances their resistance to environmental stressors and chemical treatments, complicating disease control. The key to overcoming this challenge lies in the development of multifunctional green bactericides capable of effectively breaking down biofilm barriers, improving foliar deposition properties, and achieving the control of bacterial diseases.
View Article and Find Full Text PDFBMC Genomics
November 2024
College of Life Sciences, Gannan Normal University, Ganzhou, 341000, China.
The sustainable development of the citrus industry is greatly affected by citrus canker, an important bacterial disease. To explore the transcriptional regulatory mechanism of citrus resistance to canker disease, this study used the susceptible Citrus sinensis cv. 'Newhall' and its citrus canker-resistant bud mutation variety 'Longhuitian' (LHT) as materials.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!