Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7423300 | PMC |
| http://dx.doi.org/10.1007/978-1-4939-9736-7_4 | DOI Listing |
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