A Gram-stain-negative, rod-shaped, motile, facultatively aerobic and ivory-pigmented bacterium (designated strain LA-55) was isolated from a river in the Republic of Korea. On the basis of 16S rRNA gene sequencing, strain LA-55 clustered with species of the genus and was closely related to KSL-102 (97.3 %), DSM 4731 (97.1 %), NHI-13 (97.0 %), FDRGB2b (97.0 %) and DSM 17977 (97.0 %). The average nucleotide identity value between strain LA-55 and its closest-related strain was 74.1 %, indicating that strain LA-55 represents a novel species of the genus . Growth occurred at 15-40 °C on Reasoner's 2A medium in the presence of 0-2 % NaCl (w/v) and at pH 6.0-8.0. The genomic DNA G+C content was 70.5 mol% and ubiquinone 10 (Q-10) was the major respiratory quinone. The major cellular fatty acids (>5 %) were C 6 and/or C 7 (summed feature 8), C, C 6 and/or C 7 (summed feature 3) and C 7 11-methyl. The polar lipids consisted of phosphatidylglycerol, 1,2-di--acyl-3--[d-glucopyranosyl-(1→4)--d-glucopyranuronosyl]glycerol, 1,2-di--acyl-3--d-glucopyranuronosyl glycerol, unidentified aminolipid, unidentified phosphoglycolipid and unidentified lipids. Physiological and biochemical characteristics indicated that strain LA-55 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LA-55 (=KACC 19639=LMG 30850).
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http://dx.doi.org/10.1099/ijsem.0.003736 | DOI Listing |
Int J Syst Evol Microbiol
January 2020
Department of Biotechnology, Hankyong National University, Anseong-si, Gyeonggi-do 17579, Republic of Korea.
A Gram-stain-negative, rod-shaped, motile, facultatively aerobic and ivory-pigmented bacterium (designated strain LA-55) was isolated from a river in the Republic of Korea. On the basis of 16S rRNA gene sequencing, strain LA-55 clustered with species of the genus and was closely related to KSL-102 (97.3 %), DSM 4731 (97.
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March 2014
Food Engineering, Polytechnic School, Vale do Rio dos Sinos University, Av. Unisinos, 950, São Leopoldo, RS, 93022-000, Brazil.
The aims of this research were to screen and characterize a new microbial source of γ-PGA, to optimize aspects of culture conditions and medium composition using central composite design and response surface methodologies. The influence of bioreactor stirring rates on the production of γ-PGA was also investigated and the oxygen volumetric mass transfer coefficients (k La) were established. The most productive strain was identified by 16S rDNA analysis as Bacillus subtilis, and its γ-PGA production in rotatory shaker was threefold increased under optimized conditions (37 °C, pH 6.
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Center for Red Meat Safety, Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523, USA.
The survival and growth of Listeria monocytogenes and spoilage microflora during storage of fresh beef subjected to different decontamination treatments was studied. Fresh beef inoculated with a five-strain mixture of L. monocytogenes (5.
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