d-Psicose 3-epimerase is an enzyme that catalyzes the synthesis of d-psicose from d-fructose. We cloned the d-psicose 3-epimerase from Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311. By a two-step pH regulation of segmented fermentation, we significantly improved the RDPE production and decreased the fermentation cost. The two-step regulation consisted of the first step maintained the pH value at 7.0 for 24 H and the second step adjusted the pH value up to 7.5 slowly for another 24 H. Finally, the RDPE production was increased to 74 U/mL, which was about 2.5-fold compared with the control. Our segmented fermentation strategy provides an important experimental basis for the industrial-scale production of RDPE.
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Article Synopsis
- * There are two main ways to produce D-allulose: chemical synthesis, which can create unwanted byproducts, and biosynthesis, which uses enzymes to convert starch or glycerol into D-allulose more efficiently.
- * The article reviews recent research on biosynthesis, highlighting the enzymes used, their properties, and the potential for improved production methods for D-allulose.
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