The mixed antiglobulin reaction (MAR) test for immunoglobulins G was performed on 574 spermograms, resulting in the detection of surface auto-antibodies directed against spermatozoa in 4.18% of the cases. Concomitant investigations of spermograms for spontaneous agglutination and cross-penetration in a control cervical mucus, together with assays of antispermatozoa antibodies in serum and seminal fluid, have led to the diagnosis of asthenospermia in 87.5% of the cases. Spontaneous sperm agglutination was present in all cases; the cross-penetration test was negative or doubtful in 92.3%. Auto-antibodies against spermatozoa were found in 76.2% of serum and/or seminal fluid samples.

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