Background: (Sieb.) Makino () is one species of bamboo distributed in China, Japan and Vietnam. The chemical profile of its leaves and its potential application was unknown yet.
Methods: The chemical profile of was studied by HPLC and UPLC-DAD-QTOF-MS. The extract was prepared by extraction with 50% aqueous ethanol, followed by H103 macroporous resins adsorption and desorption processes. Pancreatic lipase inhibitory activity was determined using -nitrophenyl palmitate as the substance, which was hydrolyzed by lipase to form coloured nitrophenol.
Results: Eighteen compounds were identified in . Most of them were the -glycosylated derivatives of luteolin and apigenin, such as isoorientin, isoorientin-2″--rhamnoside and isovitexin. Isoorientin-2″--rhamnoside was the most dominant flavonoid in the sample. extract was prepared through resin adsorption/desorption with yield of 1.12 ± 015% and total flavonoids content of 82 ± 2 mg/g (in term of isoorientin). The extract exhibited pancreatic lipase inhibitory activity with IC50 value of 0.93 mg/mL.
Conclusion: The chemical profile of leaves was uncovered for the first time. -glycosyl flavonoids were the main constituents in the plant. The extract exhibited pancreatic lipase inhibitory activity and may have potential for use as a food supplement for controlling obesity.
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| http://dx.doi.org/10.7717/peerj.7765 | DOI Listing |
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