Background: Probiotics are widely used in dogs but can be associated with alterations in some serum biochemistry test results.
Objective: To assess the effect of Enterococcus faecium SF68 administration for 14 days on serum alanine transferase (ALT) and alkaline phosphatase (ALP) activity and total cholesterol and triglyceride concentrations in healthy dogs.
Animals: Thirty-six healthy privately owned neutered dogs were randomly allocated, stratified by sex, to control or probiotic groups. Dogs were clinically healthy, with normal physical examination findings, blood, urine, and fecal analyses and ultrasonographic examinations.
Method: In this blinded, controlled study E. faecium SF68 was administered to the probiotic group for 14 days. Blood samples were taken from all dogs at days 0, 14, and 28. Serum ALT and ALP activity and total cholesterol and triglyceride concentrations were determined on these 3 days.
Results: The probiotic induced no significant changes in mean ALT and ALP activity. Mean cholesterol concentration did not change during probiotic administration but a significant decrease was seen on day 28 (P < .01). Mean triglyceride concentration increased progressively, becoming significant at day 28 (P < .05), with 1 dog developing hypertriglyceridemia.
Conclusions And Clinical Importance: E. faecium SF68 would not create confusion when monitoring dogs with hepatobiliary disease because ALT and ALP activity did not change significantly. A significant decrease in cholesterol and significant increase in triglyceride concentrations were seen at day 28 but were not clinically relevant, with 1 dog showing hypertriglyceridemia. A longer trial is warranted to assess if the probiotic effects could be clinically relevant and to assess its potential use in hypertriglyceridemic dogs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6872625 | PMC |
| http://dx.doi.org/10.1111/jvim.15625 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An update on the taxonomy and functional properties of the probiotic Enterococcus faecium SF68.
Benef Microbes
March 2024
Human Effective Microbes Laboratory, Graduate School of Advanced Convergence, Handong Global University, Pohang, Gyeongbuk 37554, Republic of Korea.
Enterococcus faecium SF68 (SF68) is a well-known probiotic with a long history of safe use. Recent changes in the taxonomy of enterococci have shown that a novel species, Enterococcus lactis, is closely related with E. faecium and occurs together with other enterococci in a phylogenetically well-defined E.
View Article and Find Full Text PDFDietary Supplementation with the Probiotic SF68 Reinforces Intestinal Epithelial Barrier in Obese Mice by Improving Butyrate Bioavailability.
Mol Nutr Food Res
July 2023
Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy.
Scope: Modifications in intestinal microbiota and its metabolites, the short-chain fatty acids (SCFA) are main factors altering intestinal epithelial barrier integrity and eliciting the onset of a meta-inflammation observed in obesity. The present study is aimed at evaluating the efficacy of Enterococcus faecium (SF68) administration in counteracting the impairment of gut barrier and enteric inflammation in a model of diet-induced obesity, characterizing the molecular mechanisms underlying such beneficial effects.
Methods And Results: Male C57BL/6J mice, fed with standard diet (SD) or high-fat diet (HFD), are treated with SF68 (10 CFU day ).
The administration of SF68 counteracts compositional shifts in the gut microbiota of diet-induced obese mice.
Front Microbiol
December 2022
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
Article Synopsis
- Scientists are studying probiotics, which are tiny good germs, to help prevent and treat obesity by changing the bacteria in the gut.
- In an experiment, mice on a high-fat diet were given a specific probiotic called SF68 to see if it could help them gain less weight and improve their gut bacteria.
- The results showed that mice taking SF68 gained less weight and had better gut bacteria that helped reduce inflammation and sugar levels in their bodies, making it a promising treatment for obesity.
A virulence factor as a therapeutic: the probiotic SF68 arginine deiminase inhibits innate immune signaling pathways.
Gut Microbes
August 2022
Department of Veterinary Medicine, Institute of Microbiology and Epizootics, Centre for Infection Medicine, Free University of Berlin, Berlin, Germany.
The probiotic bacterial strain SF68 has been shown to alleviate symptoms of intestinal inflammation in human clinical trials and animal feed supplementation studies. To identify factors involved in immunomodulatory effects on host cells, SF68 and other commensal and clinical isolates were screened using intestinal epithelial cell lines harboring reporter fusions for NF-κB and JNK(AP-1) activation to determine the responses of host cell innate immune signaling pathways when challenged with bacterial protein and cell components. Cell-free, whole-cell lysates of SF68 showed a reversible, inhibitory effect on both NF-κB and JNK(AP-1) signaling pathway activation in intestinal epithelial cells and abrogated the response to bacterial and other Toll-like receptor (TLR) ligands.
View Article and Find Full Text PDFNorepinephrine and Serotonin Can Modulate the Behavior of the Probiotic NCIMB10415 towards the Host: Is a Putative Surface Sensor Involved?
Microorganisms
February 2022
Laboratory of Microbial Biochemistry and Proteomics, Department of Life Sciences and Systems Biology, University of Turin, Via Accademia Albertina 13, 10123 Torino, Italy.
The human gut microbiota has co-evolved with humans by exchanging bidirectional signals. This study aims at deepening the knowledge of this crucial relationship by analyzing phenotypic and interactive responses of the probiotic NCIMB10415 ( SF68) to the top-down signals norepinephrine (NE) and serotonin (5HT), two neuroactive molecules abundant in the gut. We treated NCIMB10415 with 100 µM NE and 50 µM 5HT and tested its ability to form static biofilm (Confocal Laser Scanning Microscopy), adhere to the Caco-2/TC7 monolayer, affect the epithelial barrier function (Transepithelial Electrical Resistance) and human dendritic cells (DC) maturation, differentiation, and cytokines production.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!