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| http://dx.doi.org/10.21037/atm.2019.06.50 | DOI Listing |
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Electrophoresis-Correlative Ion Mobility Deepens Single-cell Proteomics in Capillary Electrophoresis Mass Spectrometry.
Mol Cell Proteomics
December 2024
Department of Chemistry & Biochemistry, University of Maryland, College Park, MD 20742. Electronic address:
Detection of trace-sensitive signals is a current challenge in single-cell mass spectrometry (MS) proteomics. Separation prior to detection improves the fidelity and depth of proteome identification and quantification. We recently recognized capillary electrophoresis (CE) electrospray ionization (ESI) for ordering peptides into mass-to-charge (m/z)-dependent series, introducing electrophoresis-correlative (Eco) data-independent acquisition.
View Article and Find Full Text PDFThe Chondrogenic Potential of the Bovine Tendon Sheath - a Novel Source of Stem Cells for Cartilage Repair.
Stem Cells
December 2024
Department of Plastic and Hand Surgery, Inselspital Bern University Hospital, Bern, Switzerland.
The human hand is traumatized more frequently than any other bodily part. Trauma and pathological processes (e.g.
View Article and Find Full Text PDFArticle Synopsis
- Single-cell mass spectrometry faces challenges in detecting trace-sensitive signals, but separating peptides before detection can boost the accuracy and depth of proteome analysis.
- A new method called electrophoresis-correlative data-independent acquisition (Eco) utilizes capillary electrophoresis and electrospray ionization to enhance peptide ion detection by organizing them based on their mobility.
- This technique significantly outperformed classical methods, leading to the identification of more proteins from minimal samples, demonstrating its potential in analyzing embryonic stem cells and understanding cell differentiation.
Temporomandibular Joint Fibrocartilage Contains CD105 Positive Mouse Mesenchymal Stem/Progenitor Cells with Increased Chondrogenic Potential.
J Maxillofac Oral Surg
September 2023
Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA USA.
Objective: A specific type of mesenchymal stem/progenitor cells (MSPCs), CD105 is reported to aid in cartilage regeneration through TGF-β/Smad2-signalling. The purpose of this study was to identify and characterize CD105 MSPCs in temporomandibular joint (TMJ) cartilage.
Materials And Methods: MSPCs were isolated from mouse TMJ condyle explants and evaluated for their clonogenicity and pluripotential abilities.
Stem cell pathology: histogenesis of malignant fibrous histiocytoma and characterization of myofibroblasts appearing in fibrotic lesions.
J Vet Med Sci
September 2023
Laboratory of Veterinary Pathology, Osaka Metropolitan University, Osaka, Japan.
The concept of "stem cell pathology" is to establish the role of the stem cells by exploring their contribution to lesion development. The somatic stem cells are present in the body. Malignant fibrous histiocytoma (MFH; recently named "undifferentiated pleomorphic sarcoma") includes pluripotential undifferentiated mesenchymal stem cells as a cell element.
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