AI Article Synopsis
- Human thymic epithelial monolayer-conditioned medium (TEM-CM) significantly boosted suppressor T-lymphocyte activity in pediatric cancer patients' bone marrow within a week after chemotherapy, indicating enhanced immune response (P < 0.001).
- Conversely, TEM-CM reduced T-lymphocyte activity in samples taken 14-21 days post-chemotherapy, reflecting a decline in immune function over time (P < 0.05).
- TEM-CM had no notable impact on normal subjects’ suppressor T-cell activity or on spontaneous activity in both patients and normals, highlighting distinct post-chemotherapy immune alterations.
Article Abstract
Human thymic epithelial monolayer-conditioned medium (TEM-CM) enhanced concanavalin A (ConA)-induced suppressor T-lymphocyte activity in 15 of 17 studies of fractionated light-density bone marrow mononuclear cells (LD-BMMC) obtained from pediatric cancer patients within 7 days of chemotherapy (P less than 0.001). However, TEM-CM depressed ConA-induced suppressor T-lymphocyte activity in 14 of 18 studies of LD-BMMC obtained from patients who had received their chemotherapy 14-21 days previously (P less than 0.05). In studies of LD-BMMC from normal subjects, TEM-CM did not show any significant effect on suppressor cell activity, nor did TEM-CM significantly affect spontaneous suppressor cell activity in patients or normals. The effect of direct culture on thymic epithelial monolayers was equivalent to the effect of TEM-CM in both ConA-induced and spontaneous suppressor cell assays. These data demonstrate thymic factor-mediated changes in suppressor T-cell activity of pediatric cancer patients and suggest a postchemotherapy alteration in the bone marrow population of inducible prethymic T cells.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11039190 | PMC |
| http://dx.doi.org/10.1007/BF00199725 | DOI Listing |
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