Stability of epidermal growth factor, fibronectin, and alpha-2-macroglobulin in canine serum under different storage conditions.

Can J Vet Res

Department of Clinical Sciences (Briffod, Vanore) and Department of Microbiology and Pathology (Bédard), Faculty of Veterinary Medicine, University of Montreal, 3200 rue Sicotte, St-Hyacinthe, Quebec J2S 2M2.

Published: October 2019

AI Article Synopsis

  • The study examined the stability of epidermal growth factor (EGF), fibronectin, and alpha(α)-2-macroglobulin in canine serum under different storage conditions.
  • After 1 week at 4°C, EGF levels decreased significantly, while α-2-macroglobulin levels increased; however, no changes were noted for fibronectin.
  • Overall, EGF and fibronectin remained stable for 3 months at -20°C, suggesting that storing samples at this temperature is effective for preserving these factors.

Article Abstract

The objective of this study was to assess whether concentrations of epidermal growth factor (EGF), fibronectin, and alpha(α)-2-macroglobulin in canine serum remain stable under different storage conditions. Serum was obtained from 10 adult dogs and stored for 7 d at room temperature (RT) and at 4°C and for 1, 3, and 6 mo at -20°C. Bacterial cultures of serum were carried out after 7 d at 4°C and at RT. For each dog and time point, EGF, fibronectin, and α-2-macroglobulin were quantified in duplicate by enzyme-linked immunosorbent assay (ELISA). Mean concentrations of each factor at each time point were used for statistical analysis. No bacterial growth was observed in any samples. Compared to baseline (232.24 ± 49.47 pg/mL), EGF concentration was significantly lower after 1 wk of storage at 4°C (135.39 ± 27.12 pg/mL, = 0.006), but not at RT (315.85 ± 79 pg/mL, = 0.6) or after 1, 3, or 6 mo of storage at -20°C (220.84 ± 41.07 pg/mL, = 0.7; 220.98 ± 78.26 pg/mL, = 0.8; 266.06 ± 20.39 pg/mL, = 0.4, respectively). Compared to baseline, concentrations of fibronectin after 1 wk of storage at 4°C or at RT and 1, 3, or 6 mo of storage at -20°C were not statistically different. Compared to baseline (186.67 ± 45.20 mg/dL), the concentration of α-2-macroglobulin after 1 wk of storage at 4°C was significantly increased (244.61 ± 58.27 mg/dL, = 0.002), but not at RT (177.09 ± 26.99 mg/dL, = 0.2). The differences in concentration after 3 and 6 mo of storage at -20°C were significant compared to baseline (243.32 ± 42.64 mg/dL, = 0.005 and 56.39 ± 21.78 mg/dL, < 0.0001, respectively), but not after 1 mo of storage at -20°C (136.79 ± 25.61 mg/dL, = 0.1). One week of storage at RT has little effect on the stability of EGF, fibronectin, and α-2-macroglobulin in canine serum. Measured factors remain stable for 3 mo of storage at -20°C.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6753891PMC

Publication Analysis

Top Keywords

storage -20°c
20
compared baseline
16
canine serum
12
egf fibronectin
12
storage 4°c
12
storage
11
epidermal growth
8
growth factor
8
storage conditions
8
remain stable
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!