This study is the first to examine the background level of male DNA on underpants worn by females in the absence of sexual contact. Here, we examined 103 samples from the inside front of underpants from 85 female volunteers. Samples were examined for the presence of male DNA using NGM SElect and PowerPlex Y23 kits. Only five samples gave a "complete" Y-STR profile, even though 83.5% of our volunteers cohabited with a male. In all cases where a partner reference sample was available, the Y-STR profile matched the cohabiting partner. We have demonstrated that a Y-STR profile is not expected on the inside front of underpants worn by females after social contact alone. The results of this study are informative for evaluating the significance of a Y-STR profile on underpants in cases of alleged sexual assault.
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| http://dx.doi.org/10.1111/1556-4029.14198 | DOI Listing |
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Article Synopsis
- The study evaluates methods for estimating the number of contributors (NoC) to mixed Y-STR profiles, focusing on maximum allele count (MAC) and total allele count (TAC) approaches.
- It introduces new definitions for MAC (MAC-single and MAC-multi) specific to single-copy and multi-copy loci, respectively, and analyzes a dataset of 120,000 Y-STR profiles from two different populations.
- Results indicate that both MAC-single and TAC methods demonstrate high accuracy in estimating NoC, with accuracy rates varying based on the population and the method used.
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