Heterologous Expression of Gene in Enhances Reactive Oxygen Species Production and 11α-Hydroxylation Rate of 16α, 17-Epoxyprogesterone.

The 11α-hydroxylation of 16α, 17-epoxyprogesterone (EP) catalyzed by is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The 11α-steroid hydroxylase CYP509C12 from were reported to play a crucial role in the 11α-hydroxylation in recombinant fission yeast. In the present study, the of () was introduced into . using the liposome-mediated mycelial transformation. Heterologous expression of resulted in increased fungal growth and improved intracellular reactive oxygen species content in . . The HO levels in transformants were approximately 2-folder that of the wild type (RnWT) strain, with the superoxide dismutase activities increased approximately 45% and catalase activities decreased approximately 68%. Furthermore, the 11α-hydroxylation rates of EP in transformants (C4, C6 and C9) were 39.7%, 38.3% and 38.7%, which were 12.1%, 8.2% and 9.4% higher than the rate of the RnWT strain, respectively. This paper investigated the effect of heterologous expression of in , providing an effective genetic method to construct the engineered strains for steroid industry.