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Measurement of oxidatively damaged DNA in mammalian cells using the comet assay: Reflections on validity, reliability and variability.
Mutat Res Genet Toxicol Environ Mutagen
January 2022
Section of Environmental Health, Department of Public Health, University of Copenhagen, Øster Farimagsgade 5A, DK-1014, Copenhagen K, Denmark. Electronic address:
The comet assay is a simple technique for measurements of low levels of DNA damage and repair in single cells. However, there is variation in background levels of DNA damage in peripheral blood mononuclear cells (PBMCs). This variation has been documented by inter-laboratory ring-trials where identical samples have been analysed in different laboratories using the formamidopyrimidine DNA glycosylase (Fpg)-modified comet assay.
View Article and Find Full Text PDFIntroduction to hCOMET special issue, 'Comet assay in vitro'.
Mutat Res Genet Toxicol Environ Mutagen
September 2019
Department of Nutrition, Institute for Basic Medical Sciences, University of Oslo, Oslo, Norway.
Crystal and molecular structure of two geometrically restricted chemotactic tripeptides, analogues of formyl-methionine-leucine-phenylalanine.
Int J Pept Protein Res
December 1990
Laboratory of Structural Chemistry and Molecular Modelling, University of Sherbrooke, Quebec, Canada.
The crystal structures of HCO-Met-Leu-Phe-OC(CH3)3, (CH25H39N3O5S), fMLP-OtBu, and HCO-Met psi [CSNH]-Leu-Phe-OCH3, (C22H33N3O4S2), fMS LP-OMe, have been determined by single crystal X-ray diffraction, and their conformational properties investigated by molecular mechanics energy calculations. Crystals of fMLP-OtBu are monoclinic, space group P2(1), a = 12.027(4), b = 9.
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