AI Article Synopsis
- A new sucrose transport assay has been developed using a fluorescence method instead of traditional radioactive techniques, making it more convenient for lab use.
- Researchers introduced a potato sucrose transporter into strains and confirmed its functionality through this new assay.
- This methodology can enhance understanding and engineering of sucrose transport, potentially improving sucrose fermentation processes in these strains.
Article Abstract
A convenient and effective sucrose transport assay for strains is needed. Traditional methods, such as C-sucrose isotope labelling, use radioactive materials and are not convenient for many laboratories. Here, a sucrose transporter from potato was introduced into , and a fluorescence assay based on esculin was used for the analysis of sucrose transport in strains. This showed that the heterologously expressed potato sucrose transporter is functional in . Recombinant engineering of high-level sucrose transport would aid sucrose fermentation in strains. The assay described herein provides an important technological platform for studying sucrose transporter function following heterologous expression in .
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6803915 | PMC |
| http://dx.doi.org/10.3390/molecules24193495 | DOI Listing |
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