Microbiota of MR1 deficient mice confer resistance against Clostridium difficile infection.

PLoS One

Laboratory of Mucosal Pathogens and Cellular Immunology, Division of Bacterial Pathogens and Allergenic Products, Office of Vaccines Research and Review, Center for Biologics Evaluation and Research, United States Food and Drug Administration, Silver Spring, Maryland, United States of America.

Published: April 2020

AI Article Synopsis

  • Clostridium difficile infection (CDI) often follows antibiotic use, which disrupts the gut's microbial balance.
  • This study explored the role of Major histocompatibility complex-related protein 1 (MR1) in influencing gut immunity against CDI using wild-type and MR1-/- mice.
  • Surprisingly, MR1-/- mice showed resistance to the infection, and their unique gut microbiome could be transferred to wild-type mice through fecal microbiota transplantation, indicating that MR1 affects gut flora and CDI vulnerability.

Article Abstract

Clostridium difficile (Cd) infection (CDI) typically occurs after antibiotic usage perturbs the gut microbiota. Mucosa-associated invariant T cells (MAIT) are found in the gut and their development is dependent on Major histocompatibility complex-related protein 1 (MR1) and the host microbiome. Here we were interested in determining whether the absence of MR1 impacts resistance to CDI. To this end, wild-type (WT) and MR1-/- mice were treated with antibiotics and then infected with Cd spores. Surprisingly, MR1-/- mice exhibited resistance to Cd colonization. 16S rRNA gene sequencing of feces revealed inherent differences in microbial composition. This colonization resistance was transferred from MR1-/- to WT mice via fecal microbiota transplantation, suggesting that MR1-dependent factors influence the microbiota, leading to CDI susceptibility.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6764671PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0223025PLOS

Publication Analysis

Top Keywords

mr1-/- mice
12
clostridium difficile
8
difficile infection
8
microbiota
4
microbiota mr1
4
mr1 deficient
4
mice
4
deficient mice
4
mice confer
4
resistance
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!