Aim: To evaluate biological behavior of human peripheral mononuclear cells (PBMC) in contact with porous tantalum (PT) and Porphyromonas gingivalis (Pg).
Methods: Pg was incubated for 8 hours. The groups formed were: PBMC (control), PBMC + PT, PBMC + Pg and PBMC + PT + Pg. Cell viability was evaluated using MTT assay. The morphology and adhesion of PBMC to PT was evaluated using scanning electron microscopy. Expression of interleukin (IL)-10, transforming growth factor (TGF)-β, matrix metallopeptidase (MMP)-9 and receptor activator of nuclear factor-κΒ ligand (RANKL) was determined by enzyme-linked immunosorbent assay.
Results: MTT assay revealed that PT did not interfere in the mitochondrial activity of PBMC (P > .05). Scanning electron microscopy showed the adherence of PBMC to PT. IL-10 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg. TGF-β levels in PBMC + PT were higher than PBMC and PBMC + Pg. MMP-9 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg and PBMC + PT + Pg. RANKL levels in PBMC + PT were lower than in PBMC.
Conclusion: PT did not affect PBMC viability, allowed cell adhesion, reduced expression of RANKL and enhanced TGF-β in comparison with the control group.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/jicd.12472 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Phenotypic screening in zebrafish larvae identifies promising cyanobacterial strains and pheophorbide a as insulin mimetics.
Sci Rep
December 2024
Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/CIMAR), University of Porto, Avenida General Norton de Matos, s/n, Matosinhos, 4450-208, Portugal.
Diabetes is a pandemic disease that causes the loss of control of glucose regulation in the organism, in consequence of dysfunction of insulin production or functionality. In this work, the antidiabetic bioactivity of 182 fractions from 19 cyanobacteria strains derived from the LEGE Culture Collection were analysed using the 2-NBDG assay in zebrafish larvae. From this initial screening, two fractions (57 (06104_D) and 107 (03283_B)) were identified as promising insulin mimetics.
View Article and Find Full Text PDFEvaluating Self-Directed Rehabilitation for Knee and Hip Arthroplasty During the COVID-19 Pandemic: A Multicenter Study.
Med Sci (Basel)
November 2024
Orthopedic and Fracture Specialists, Portland, OR 97225, USA.
The COVID-19 pandemic has triggered the adoption of new technologies to reduce the need for in-person physical therapy (PT). This study evaluated the impact of the COVID-19 pandemic on PT utilization and outcomes of patients prescribed a smartphone-based care management platform (sbCMP) for self-directed rehabilitation (SDR). A secondary analysis of data collected in a multicenter, prospective cohort trial investigating a mobile platform to deliver SDR after arthroplasty was performed.
View Article and Find Full Text PDFChange in standing acetabular orientation 2 years postoperatively after surgical correction of adult spinal deformity.
Orthop Traumatol Surg Res
December 2024
Arts et Métiers ParisTech, 151 Boulevard de l'Hôpital, 75013 Paris, France.
Introduction: Although sagittal alignment is known to influence pelvic position, few studies accurately identify the relationship between sagittal alignment and acetabular orientation. We hypothesized that postoperative PT should be correlated with acetabular change in native hips after surgical correction of adult spinal deformity. The objective of this study was therefore to describe the correlation between the change in pelvic tilt and the change in acetabular orientation two years after surgical correction of adult spinal deformity.
View Article and Find Full Text PDFMechanism of exacerbation of traumatic brain injury under warfarin anticoagulation in male mice.
PLoS One
December 2024
Department of Forensic Medicine, School of Medicine, Tokyo Women's Medical University, Tokyo, Japan.
Introduction: Traumatic brain injury (TBI) is exacerbated in patients on antithrombotic medications, with warfarin leading to increased bleeding in some cases. However, the extent to which this bleeding increases lethality and its long-term effects remain unclear. This study aimed to investigate the exacerbation of TBI by warfarin treatment and comprehensively evaluate the impact of TBI on the anticoagulant effects of warfarin.
View Article and Find Full Text PDFImpacts of UV-filter pollution and low pH: Sperm and adult biomarkers in the mussel Mytilus galloprovincialis in a multi-stressor context.
J Hazard Mater
January 2025
Centre for Environmental and Marine Studies (CESAM) & Department of Biology, University of Aveiro, 3810-193, Aveiro, Portugal. Electronic address:
Article Synopsis
- Understanding the combined effects of multiple environmental stressors, like UV-filters and ocean acidification, on marine species is crucial for addressing ecological challenges.
- The study focused on two UV-filters (4-MBC and BP-3) and their impacts on the reproductive health and performance of mussels under different pH levels, with findings indicating that low pH significantly worsened the effects of these contaminants.
- Results showed that while sperm cells could adapt somewhat to low pH, adult mussels faced severe physiological and biochemical declines, emphasizing the need for further research on long-term consequences for mussel populations and their ecosystems.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!