The novel dipstick DNA extraction method was tested for its reliability and usability for biological barcoding in comparison to a commercial kit and to a simplified isopropanol precipitation method using crayfish gill tissue. Following DNA extraction, the mitochondrial COI-gene was amplified in a PCR-reaction using a standard set of universal invertebrate primers. All three extraction techniques resulted in successful amplifications. With the dipstick method, PCR immediately follows the very brief DNA extraction technique. We suggest that the dipstick method is an affordable, efficient, and reliable DNA extraction method uniquely suited for biological barcoding that results in reliable and reproducible amplification for downstream applications such as sequencing. Additional tests on crayfish with primers for different parts of the mitochondrial genome and on fish using specific fish COI-primers confirmed these findings. Due to the few steps involved in the DNA extraction procedure the dipstick technique is also highly recommended for high school and university biology courses.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11033-019-05083-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Glutaraldehyde functionalized reduced graphene oxide based resistive sensors for detection of PCA3.
J Mater Chem B
January 2025
Department of Electrical, Electronics and Communication Engineering, Indian Institute of Technology Dharwad, Karnataka - 580011, India.
Prostate cancer antigen 3 (PCA3) has emerged as a critical biomarker for the early detection of prostate cancer, complementing the traditional prostate-specific antigen (PSA) testing. This research presents a novel resistive sensor based on reduced graphene oxide (RGO) functionalized with glutaraldehyde (GA)/complementary single-stranded DNA (ss-DNA) for the detection of the PCA3 RNA. The device was meticulously characterized at each fabrication step to confirm the successful integration of the various layers on the sensor device, utilizing atomic force microscopy (AFM) which confirmed the increase in the thickness of the sensor from ∼1.
View Article and Find Full Text PDFFirst Report of Causing Leaf Spot on in a greenhouse in New York State, U.S.A.
Plant Dis
January 2025
USDA-ARS , Ithaca, United States.
, commonly known as the "Chinese hibiscus", is a widely cultivated shrub with ornamental and medicinal applications (Jadhav et al., 2009). However, it is known to be susceptible to a range of pathogens including bacteria (Chase, 1986).
View Article and Find Full Text PDFGenotyping as Part of Routine Clinical Care-The Outcomes for a Large Paediatric Vascular Anomaly Cohort.
Am J Med Genet A
January 2025
Genetic Health Queensland, Royal Brisbane and Women's Hospital, Herston, Australia.
We describe the phenotypic and genotypic spectrum of patients with vascular anomaly (VA) in a paediatric multi-disciplinary VA clinic. We measured the clinical utility of genotyping by comparing pre and posttest diagnosis and management. A 46-month retrospective analysis occurred for 250 patients offered genetic testing in the VA clinic.
View Article and Find Full Text PDFDiagnosis of Leptomeningeal Disease in Diffuse Midline Gliomas by Detection of H3F3A K27M Mutation in Circulating Tumor DNA of Cerebrospinal Fluid.
Pediatr Blood Cancer
January 2025
Department of Neurosurgery, Brain Research Institute, Niigata University, Niigata, Japan.
Introduction: Leptomeningeal disease (LMD) in diffuse midline gliomas (DMGs) can lead to devastating symptoms such as severe pain, urinary incontinence, and tetraparesis, with limited treatment options. We determined whether detecting H3F3A K27M-mutant droplets in cerebrospinal fluid (CSF) circulating tumor deoxyribonucleic acid (ctDNA) could be a biomarker for detecting LMD in DMGs.
Methods: Twenty-five CSF samples were obtained from 22 DMG patients.
Extracting and analyzing micronuclei from mouse two-cell embryos fertilized with freeze-dried spermatozoa.
Commun Biol
January 2025
Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi, Japan.
Abnormal chromosome segregation (ACS) in preimplantation embryos causes miscarriages. For a normal pregnancy, it is necessary to reduce ACS occurrences in embryos. However, the causes of such abnormalities are unclear because no method to extract the segregated chromosomes from the blastomeres for detailed analysis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!