Prediction models for evaluating heavy metal uptake by L. in soil amended with sewage sludge.

The present study aims to develop prediction models for estimating the potential uptake of 10 heavy metals (HMs) (cadmium, Cd; cobalt, Co; chromium, Cr; copper, Cu; iron, Fe; manganese, Mn; molybdenum, Mo; nickel, Ni; lead, Pb; zinc, Zn) by the tissues of (root, shoot and pod) grown in soil amended with sewage sludge (SS) under greenhouse conditions. Soil organic matter (OM) was estimated by loss-on-ignition at 550 °C for 2 h. The pH was determined by shaking the soil and pure water at a 1:5 ratio. For HM quantifications, 0.5-1.0 g of each soil or plant sample was digested using a tri-acid mixture digestion method. The quantities of selected HMs were estimated by means of inductively coupled plasma optical emission spectrometry. Bio-concentration (BCF) and translocation (TF) factors were <1 for most of the HMs. In addition, simple linear correlations were significantly negative between the BCF of all studied HMs and soil pH, except for Pb, Mn and Ni, whereas significant positive correlations were observed between BCFs and soil OM, except for Mn, Ni and Zn. The accumulation of the 10 HMs in tissues was predicted using regression models based on the values of the same HM in the soil as well as its pH and OM. The calculated prediction models performed well for most HMs in tissues (except Ni in the pod, Cd in the shoot and Mn in the root). All measured soil factors (HM, pH and OM) consistently contributed to HM concentrations in the three tissues of the studied plants. These models may help to evaluate the safe cultivation of this species in soil amended with SS.