The metabolic inhibition (MI) test is a classic test for the identification of mycoplasmas, used for measuring the growth-inhibiting antibodies directed against acid-producing mycoplasmas, although their mechanism still remains obscure. To determine the major antigens involved in the immune killing of , we used a pulldown assay with anti- antibodies as bait and identified nine major antigens. Among these antigens, we performed the MI test and determined that the growth of could be inhibited effectively in the presence of complement by antibodies against specifically membrane protein P81 or UgpB in the presence of complement. Using a complement killing assay, we demonstrated that can be killed directly by complement and that antibody-dependent complement-mediated killing is more effective than that by complement alone. Complement lysis and scanning electron microscopy results revealed rupture in the presence of complement. Together, these results suggest that the metabolic inhibition of is antibody-dependent complement-mediated killing. This study provides new insights into mycoplasma killing by the complement system and may guide future vaccine development studies for the treatment of mycoplasma infection. Furthermore, our findings also indicate that mycoplasmas may be an appropriate new model for studying the lytic activity of membrane attack complex (MAC).
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6867846 | PMC |
http://dx.doi.org/10.1128/IAI.00740-19 | DOI Listing |
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