AI Article Synopsis
Article Abstract
This work reviews the field of DNA biosensors based on electrochemical determination of nanoparticle labels. These labeling platforms contain the attachment of metal nanoparticles (NPs) or quantum dots (QDs) on the target DNA or on a biorecognition reporting probe. Following the development of DNA bioassay, the nanotags are oxidized to ions, which are determined by voltammetric methods, such as pulse voltammetry (PV) and stripping voltammetry (SV). The synergistic effects of NPs amplification (as each nanoprobe releases a large number of detectable ions) and the inherent sensitivity of voltammetric techniques (e.g., thanks to the preconcentration step of SV) leads to the construction of ultrasensitive, low cost, miniaturized, and integrated biodevices. This review focuses on accomplishments in DNA sensing using voltammetric determination of nanotags (such as gold and silver NPs, and Cd- and Pb-based QDs), includes published works on integrated three electrode biodevices and paper-based biosystems, and discusses strategies for multiplex DNA assays and signal enhancement procedures. Besides, this review mentions the electroactive NP synthesis procedures and their conjugation protocols with biomolecules that enable their function as labels in DNA electrochemical biosensors.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836269 | PMC |
| http://dx.doi.org/10.3390/nano9101361 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The role of DNA nanotechnology in medical sensing.
Anal Methods
December 2024
Troy High School, 2200 Dorothy Ln, Fullerton, CA 92831, USA.
This paper explores how DNA nanotechnology enhances biosensors in medicine and pharmacology by taking advantage of the unique characteristics of DNA and the unique advantages of DNA origami technology. DNA origami allows the establishment of complex nanoobjects with precise size and complete molecular writability as well as the possibility of seamless integration and biocompatibility with biological systems. Utilizing this, the chemical denaturation of DNA chains allows for the combination of various functions, including organic fluorescence groups and photoreaction elements, This has allowed DNA origami to become a transformative tool in biotechnology and other fields because of its versatility, use in innovative applications improving the design and function of biosensors, and potential to provide greater possibilities for early disease diagnosis and personalized medicine.
View Article and Find Full Text PDFMultiple Cross Displacement Amplification Combined with Real-Time Fluorescence Monitoring for Efficient, Specific, and Sensitive Detection.
ACS Omega
December 2024
Experiment Research Center, Capital Institute of Pediatrics, Beijing 100020, PR China.
Invasive meningococcal disease, caused by (), is a critical global health issue, necessitating swift and precise diagnostics for effective management and control. Here, we introduce a novel diagnostic assay, NM-RT-MCDA, that combines multiple cross displacement amplification (MCDA) with real-time fluorescence detection, targeting a specific gene region in the genome. The assay utilizes a primer set designed for high specificity and incorporates a fluorophore-quencher pair with a restriction endonuclease site for real-time monitoring.
View Article and Find Full Text PDFColorimetric Hybridization Sensor for DNA Mimic of a SARS-CoV-2 RNA Marker: Direct and Inverse Bioanalysis.
ACS Meas Sci Au
December 2024
Department of Chemistry, Oklahoma State University, Stillwater, Oklahoma 74078, United States.
This article presents a colorimetric visual biosensor designed for direct application in undiluted biofluids, which holds significant promise for point-of-need applications. Unlike traditional biosensors that struggle with heavily diluted sample matrices, the presented biosensor does not require any instrumentation or trained personnel, making it highly practical. The sensor features an oligonucleotide probe covalently attached to magnetically separable magnetite (FeO) particles.
View Article and Find Full Text PDFTailoring DNA Surface Interactions on Single-Layer Graphene: Comparative Analysis of Pyrene, Acridine, and Fluorenyl Methyl Linkers.
Langmuir
December 2024
International Iberian Nanotechnology Laboratory, 4715-330 Braga, Portugal.
This study investigates the effect of different linkers and solvents on the immobilization of DNA probes on graphene surfaces, which are crucial for developing high-performance biosensors. Quartz crystal microbalance with dissipation (QCM-D) measurements were used to characterize in situ and real-time the immobilization of ssDNA and hybridization efficiency on model graphene surfaces. The DNA probes immobilization kinetics and thermodynamics were systematically investigated for all the pairings between three bifunctional linkers─1-pyrenebutyric acid succinimidyl ester (PBSE), Fluorenylmethylsuccinimidyl carbonate (FSC), and Acridine Orange (AO) succinimidyl ester─and three organic solvents (DMF, DMSO, and 10% DMF/ethanol).
View Article and Find Full Text PDFAn electrochemical aptasensor for the detection of bisphenol A based on triple signal amplification assisted by gold nanoparticles, hemin/G-quadruplex DNAzyme, and exonuclease I.
Mikrochim Acta
December 2024
Key Laboratory for Analytical Science of Food Safety and Biology, MOE, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350108, China.
A triple signal amplified electrochemical aptasensor for the detection of bisphenol A (BPA) was developed for the first time based on gold nanoparticles (AuNPs), hemin/G-quadruplex DNAzyme, and exonuclease I (Exo I) assisted amplification strategies. The BPA aptamer (Apt) hybridized with the capture probe (CP) was fixed on the gold electrode (GE) to form the double-stranded DNA (dsDNA) structure. When BPA was present, the Apt was detached from the GE surface by specific recognition between the BPA and Apt, forming BPA-Apt complexes in solution.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!