AI Article Synopsis
- This study shows that pectin from Spirulina maxima (SmP) boosts disease resistance in zebrafish, with higher survival rates against harmful bacteria compared to the control group.
- Zebrafish larvae treated with SmP demonstrated significant increases in immune response genes, enhancing their defense mechanisms.
- Overall, SmP appears to be a safe, natural option for improving immune responses and promoting disease resistance in aquaculture.
Article Abstract
In this study, we demonstrate the enhanced disease resistance and positive immunomodulation of novel pectin isolated from Spirulina maxima (SmP) in zebrafish model. Zebrafish larvae exposed to SmP had significantly (p < 0.05) higher cumulative percent survival (CPS) at 25 (44.0%) and 50 μg/mL (67.0%) against Edwardsiella piscicida compared to the control. However, upon Aeromonas hydrophila challenge, SmP exposed larvae at 50 μg/mL had slightly higher CPS (33.3%) compared to control group (26.7%). SmP supplemented zebrafish exhibited the higher CPS against E. piscicida (93.3%) and A. hydrophila (60.0%) during the early stage of post-infection (<18 hpi). qRT-PCR results demonstrated that exposing (larvae) and feeding (adults) of SmP, drive the modulation of a wide array of immune response genes. In SmP exposed larvae, up-regulation of the antimicrobial enzyme (lyz: 3.5-fold), mucin (muc5.1: 2.84, muc5.2: 2.11 and muc5.3: 2.40-fold), pro-inflammatory cytokines (il1β: 1.79-fold) and anti-oxidants (cat: 2.87 and sod1: 1.82-fold) were identified. In SmP fed adult zebrafish (gut) showed >2-fold induced pro-inflammatory cytokine (il1β) and chemokines (cxcl18b, ccl34a.4 and ccl34b.4). Overall results confirmed the positive modulation of innate immune responses in larval stage and it could be the main reason for developing disease resistance against E. piscicida and A. hydrophila. Thus, non-toxic, natural and biodegradable SmP could be considered as the potential immunomodulatory agent for sustainable aquaculture.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.fsi.2019.09.054 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Folic acid-targeted β-lactoglobulin nanocarriers for enhanced delivery of 5-fluorouracil and sodium butyrate in colorectal cancer treatment.
Int J Pharm
January 2025
Department of Physics, Kharazmi University, Tehran, Iran; Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran. Electronic address:
Colorectal cancer (CRC) remains a significant public health concern, emphasizing the need for innovative therapeutic strategies to improve patient outcomes. This study aimed to develop a highly efficient nanocarrier for targeted drug delivery, enhancing drug efficacy while minimizing concentrations and limiting adverse effects. We synthesized protein-based β-lactoglobulin (βlg) nanoparticles (NPs), loaded with 5-fluorouracil (5-FU) and sodium butyrate (NaB), and further functionalized with folic acid (FA) for specific targeting of folate receptor-positive CRC cells.
View Article and Find Full Text PDFOptimizing Non-Thermal Magnetic Field to Minimize Weight Loss and Tissue Degradation: Identifying Possible Enzyme Inhibition Mechanisms.
Foods
January 2025
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung City 402202, Taiwan.
This research investigates potential mechanisms of novel magnetic field (MF) treatments in inhibiting cell-wall-degrading enzymes, aiming to reduce weight loss and preserve the post-harvest quality of tomatoes ( L.) as a climacteric fruit. The optimization of the processing parameters, including MF intensity (1, 2, 3 mT), frequency (0, 50, 100 Hz), and duration (10, 20, 30 min), was accomplished by applying an orthogonal array design.
View Article and Find Full Text PDFThe Effects of Cold-Plasma Technology on the Quality Properties of Fresh-Cut Produce: A Review.
Foods
January 2025
College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
With improving economic conditions, consumer demand for fresh-cut produce is rising. The development of the fresh-cut industry has been hindered by pathogenic contamination and quality deterioration. Scientific communities have developed novel preservation technologies for fresh-cut produce.
View Article and Find Full Text PDFPrediction and Validation of New Targets of NNPCN in Inhibiting Based on Molecular Docking, Dynamics, and Biotechnology.
J Agric Food Chem
January 2025
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China.
The antifungal targets of the new fungicide -(naphthalen-1-yl)-phenazine-1-carboxamide (NNPCN) are still incomplete, limiting its application. To identify potential new targets of NNPCN and facilitate target hunting, a suite of techniques was employed to conduct experiments on . Nine potential targets were identified, exhibiting strong binding affinity to NNPCN, as indicated by binding free energies below -100.
View Article and Find Full Text PDFPreparation, structural property, and antioxidant activities of a novel pectin polysaccharide from the flowers of Linn.
Front Nutr
January 2025
Prefabricated Dish Industry Development Research Institute, Zhejiang Dong Fang Polytechnic, Wenzhou, China.
Introduction: Oxidative stress, triggered by an imbalance between reactive oxygen species (ROS) production and cellular antioxidant defense mechanisms, is implicated in various pathological conditions. Plant-derived polysaccharides have gained significant attention as potential natural antioxidants due to their biocompatibility, biodegradability, and structural versatility.
Methods: This study focuses on the purification, structural characterization, and antioxidant activities of a novel pectin polysaccharide (HFPS) isolated from the flowers of Linn.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!