Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 1034
Function: getPubMedXML
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3152
Function: GetPubMedArticleOutput_2016
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
The detection of oxidative stress caused by emerging pollutants in aquatic systems is essential to carry out toxicological analysis since they can bring us information about the mechanisms of toxic action of the pollutants, which might be useful to address this contamination. To achieve this goal, two self-bioluminescent strains that respond to oxidative stress based on the filamentous cyanobacterium Nostoc sp. PCC7120, which has a high ecological relevance in aquatic continental systems, have been constructed. Nostoc sp. PCC7120 pBG2172 harbours the promoter region of the 2-cys-prx gene (P2-cys-prx), encoding a cytoplasmic peroxiredoxin, fused to luxCDABE genes of the bacterium Photorhabdus luminescens. Nostoc sp. PCC7120 pBG2173 harbours the promoter region of the KatA gene (PkatA), a cytoplasmic catalase, also fused to luxCDABE genes. Both strains have been characterized by exposing them to HO: Nostoc sp. PCC7120 pBG2172 responded while Nostoc sp. PCC7120 pBG2173 did not respond to this pollutant. In order to know their specificity, they were exposed to methyl viologen (MV), an herbicide that produces superoxide anion (O) and a bioluminescence response was observed in both strains. Besides, the utility of these strains for the detection of HO and MV in natural water samples, both pristine and wastewater samples has been tested by spiking experiments. Finally, the possible application of these strains for the detection of the emerging pollutant triclosan has also been tested showing to be suitable bioreporters to study oxidative stress in aquatic environments.
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Source |
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http://dx.doi.org/10.1016/j.chemosphere.2019.124395 | DOI Listing |
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