AI Article Synopsis
- The DNA cytosine deaminase APOBEC3B (A3B) is important for the immune response but also linked to cancer development in various solid tumors.
- Researchers developed a new rabbit monoclonal antibody, 5210-87-13, that is effective for various applications like ELISA and immunohistochemistry.
- This antibody stands out for accurately detecting native A3B in tumor tissues, suggesting it could enhance studies on A3B’s role in human health and disease.
Article Abstract
The DNA cytosine deaminase APOBEC3B (A3B) is normally an antiviral factor in the innate immune response. However, A3B has been implicated in cancer mutagenesis, particularly in solid tumors of the bladder, breast, cervix, head/neck, and lung. Here, we report data on the generation and characterization of a rabbit monoclonal antibody (mAb) for human A3B. One mAb, 5210-87-13, demonstrates utility in multiple applications, including ELISA, immunoblot, immunofluorescence microscopy, and immunohistochemistry. In head-to-head tests with commercial reagents, 5210-87-13 was the only rabbit monoclonal suitable for detecting native A3B and for immunohistochemical quantification of A3B in tumor tissues. This novel mAb has the potential to enable a wide range of fundamental and clinical studies on A3B in human biology and disease.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6783943 | PMC |
| http://dx.doi.org/10.3390/antib8030047 | DOI Listing |
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