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Regulation and Function of C-Type Natriuretic Peptide (CNP) in Gonadotrope-Derived Cell Lines. | LitMetric

AI Article Synopsis

  • - C-type natriuretic peptide (CNP) plays a key role in regulating growth, metabolism, and reproduction, primarily in the central nervous system and pituitary gland, where it interacts with gonadotropin-releasing hormone (GnRH) signaling in gonadotrope cells.
  • - This study investigates how continuous and pulsatile GnRH stimulation affects CNP expression in specific gonadotrope cell lines (LβT2 and αT3-1), revealing that pulsatile GnRH increases certain natriuretic peptide expressions, while continuous exposure does not.
  • - The results indicate that CNP influences the expression of genes related to gonadotrope function, suggesting it may provide additional regulatory mechanisms for reproductive processes

Article Abstract

C-type natriuretic peptide (CNP) is the most conserved member of the mammalian natriuretic peptide family, and is implicated in the endocrine regulation of growth, metabolism and reproduction. CNP is expressed throughout the body, but is particularly abundant in the central nervous system and anterior pituitary gland. Pituitary gonadotropes are regulated by pulsatile release of gonadotropin releasing hormone (GnRH) from the hypothalamus, to control reproductive function. GnRH and CNP reciprocally regulate their respective signalling pathways in αT3-1 gonadotrope cells, but effects of pulsatile GnRH stimulation on CNP expression has not been explored. Here, we examine the sensitivity of the natriuretic peptide system in LβT2 and αT3-1 gonadotrope cell lines to continuous and pulsatile GnRH stimulation, and investigate putative CNP target genes in gonadotropes. Multiplex RT-qPCR assays confirmed that primary mouse pituitary tissue express (encoding CNP and guanylyl cyclase B (GC-B), respectively) and (a CNP processing enzyme), but failed to express transcripts for or (encoding ANP and BNP, respectively). Pulsatile, but not continuous, GnRH stimulation of LβT2 cells caused significant increases in and expression within 4 h, but failed to alter natriuretic peptide gene expression in αT3-1 cells. CNP enhanced expression of , , and , within 8 h in LβT2 cells, but inhibited expression in αT3-1 cells. Collectively, these data show the gonadotrope natriuretic peptide system is sensitive to pulsatile GnRH signalling, and gonadotrope transcription factors are putative CNP-target genes. Such findings represent additional mechanisms by which CNP may regulate reproductive function.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6769446PMC
http://dx.doi.org/10.3390/cells8091086DOI Listing

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