Involvement of glutathione and glutathione metabolizing enzymes in tolerance to arsenite.

Glutathione is essential for plant tolerance to arsenic but few studies have focused on the coordination between the enzymes involved in its metabolism. We exposed to four treatments (control, 5, 10 and 20 µM As) for 24 h to evaluate the role of glutathione metabolism in arsenic response and determined the arsenic uptake, growth, membrane integrity, glutathione concentration and enzyme activities (γ-glutamyl-cysteine synthetase, glutathione reductase, glutathione peroxidase, and glutathione-S-transferase). Despite absorbing high concentrations of As, plants maintained growth and cell membrane integrity when exposed to concentrations of up to 10 µM As. The maintenance of these parameters involved glutathione concentration increase due to an increase in its biosynthetic pathway (higher γ-glutamyl-cysteine synthetase). In addition, an increase in the activity of glutathione reductase, glutathione peroxidase and glutathione-S-transferase also contributed to the conserve the cellular homeostasis. However, at the concentration of 20 µM As, the high toxicity of As affected glutathione concentration and glutathione metabolizing enzymes activities, which resulted in drastic decrease in growth and damage to cell membranes. These results showed that not only the glutathione concentration but also the coordination of the enzymes involved in the synthesis, oxidation and reduction pathways of glutathione is essential for As tolerance.