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Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches. | LitMetric
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Atomic force microscopy-based single-molecule force spectroscopy detects DNA base mismatches.

Wenjing Liu Yourong Guo Kaizhe Wang Xingfei Zhou Ying Wang Junhong Lü Zhifeng Shao Jun Hu Daniel M Czajkowsky Bin Li

Nanoscale

Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. and Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, China.

Published: October 2019

Atomic force microscopy-based single-molecule-force spectroscopy is limited by low throughput. We introduce addressable DNA origami to study multiple target molecules. Six target DNAs that differed by only a single base-pair mismatch were clearly differentiated a rupture force of only 4 pN.

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 Source
http://dx.doi.org/10.1039/c9nr05234hDOI Listing

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